Bruce Conklin, MD

Professor, Medicine

Dr. Conklin uses patient-derived induced pluripotent stem cells (iPSCs) to model potential CRISPR therapies in cardiomyocytes and neurons. Dominant mutations are particularly promising targets since precise CRISPR inactivation of disease-causing alleles could provide a cure.  Allele specific gene inactivation could be beneficial to a wide range of dominant genetic diseases.

Address: 
35 Medical Center Way, Rm 001
UCSF Box 1230
San Francisco, CA 94143
United States
Phone: 
+1 415 734-2000
Faculty Type: 
Affiliate Faculty
Publications: 

Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with BAG3 P209L myofibrillar myopathy-6.

Stem cell research

Kerstin Filippi, Isabelle Riße, Luke M. Judge, Bruce R. Conklin, Bernd K. Fleischmann, Michael Hesse

Dual a-globin-truncated erythropoietin receptor knockin restores hemoglobin production in a-thalassemia-derived erythroid cells.(link is external)

Cell reports

Chu SN, Soupene E, Sharma D, Sinha R, McCreary T, Hernandez B, Shen H, Wienert B, Bowman C, Yin H, Lesch BJ, Jia K, Romero KA, Kostamo Z, Zhang Y, Tran T, Cordero M, Homma S, Hampton JP, Gardner JM, Conklin BR, MacKenzie TC, Sheehan VA, Porteus MH, Cromer MK

Haplotype editing with CRISPR/Cas9 as a therapeutic approach for dominant-negative missense mutations in NEFL.(link is external)

bioRxiv : the preprint server for biology

Dua PH, Simon BMJ, Marley CBE, Feliciano CM, Watry HL, Steury D, Abraham A, Gilbertson EN, Ramey GD, Capra JA, Conklin BR, Judge LM

Reversal of C9orf72 mutation-induced transcriptional dysregulation and pathology in cultured human neurons by allele-specific excision.

Alzheimer's & Dementia

Aradhana Sachdev, Kamaljot Gil, Maria Sckaff, Alisha M Birk, Olubankole Aladesuyi Arogundade, Katherine A Brown, Runvir S Chouhan, Patrick Issagholian Lewin, Esha Patel, Kathleen Keough, Bruce Conklin, Alec ST Smith, Claire Clelland

Programmable epigenome editing by transient delivery of CRISPR epigenome editor ribonucleoproteins.(link is external)

bioRxiv : the preprint server for biology

Xu D, Besselink S, Ramadoss GN, Dierks PH, Lubin JP, Pattali RK, Brim JI, Christenson AE, Colias PJ, Ornelas IJ, Nguyen CD, Chasins SE, Conklin BR, Nuñez JK

Neuronal DNA repair reveals strategies to influence CRISPR editing outcomes.(link is external)

bioRxiv : the preprint server for biology

Ramadoss GN, Namaganda SJ, Hamilton JR, Sharma R, Chow KG, Macklin BL, Sun M, Liu JC, Fellmann C, Watry HL, Jin J, Perez BS, Sandoval Espinoza CR, Matia MP, Lu SH, Judge LM, Nussenzweig A, Adamson B, Murthy N, Doudna JA, Kampmann M, Conklin BR

Permittivity tensor imaging: modular label-free imaging of 3D dry mass and 3D orientation at high resolution.(link is external)

Nature methods

Yeh LH, Ivanov IE, Chandler T, Byrum JR, Chhun BB, Guo SM, Foltz C, Hashemi E, Perez-Bermejo JA, Wang H, Yu Y, Kazansky PG, Conklin BR, Han MH, Mehta SB

Advances and challenges in modeling inherited peripheral neuropathies using iPSCs.(link is external)

Experimental & molecular medicine

Van Lent J, Prior R, Pérez Siles G, Cutrupi AN, Kennerson ML, Vangansewinkel T, Wolfs E, Mukherjee-Clavin B, Nevin Z, Judge L, Conklin B, Tyynismaa H, Clark AJ, Bennett DL, Van Den Bosch L, Saporta M, Timmerman V

Dual α-globin and truncated EPO receptor knockin restores hemoglobin production in α-thalassemia-derived red blood cells.(link is external)

bioRxiv : the preprint server for biology

Chu SN, Soupene E, Wienert B, Yin H, Sharma D, McCreary T, Jia K, Homma S, Hampton JP, Gardner JM, Conklin BR, MacKenzie TC, Porteus MH, Cromer MK

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