Associate Professor
Pharmaceutical Chemistry
Research Interests
Macrophage phagocytosis, covalent inhibitor development
Faculty Type
Core CVRI Faculty
Research Summary:
Our lab takes a chemical biology and proteomics approach to develop covalent chemical tools and to study the innate immune system. We are interested in understanding, predicting, and exploiting fundamental drivers of nucleophilic amino acid reactivity. We are also exploring how macrophages interact with their environment and how these interactions can lead to cell state changes.
Publications
Macrophages transfer functional proteins to their surface during live-cell uptake.
Nature chemical biology
Integrative analysis of mRNA stability regulation uncovers a metastasis-suppressive program in breast cancer.
Science advances
An interactive resource mapping the proteome and reactive cysteine landscape across the NCI-60 reveals cell and tissue-specific profiles.
Cell chemical biology
Alveolar Type II Cell-derived MMP1 high basal cells promote destructive microcysts in idiopathic pulmonary fibrosis.
bioRxiv : the preprint server for biology
Resolving fibrosis by stimulating HSC-dependent extracellular matrix degradation.
Science translational medicine
Neutrophil TLR2 signaling promotes lipid accumulation and vascular plaque growth.
bioRxiv : the preprint server for biology
Integrative analysis of mRNA stability regulation uncovers a metastasis-suppressive program in breast cancer.
bioRxiv : the preprint server for biology
C-terminal tagging, transmembrane domain hydrophobicity, and an ER retention motif influence the secretory trafficking of the inner nuclear membrane protein emerin.
bioRxiv : the preprint server for biology
Macrophages release neuraminidase and cleaved calreticulin for programmed cell removal.
Proceedings of the National Academy of Sciences of the United States of America
Treatment of acute myeloid leukemia models by targeting a cell surface RNA-binding protein.
Nature biotechnology
Phenotypic landscape of a fungal meningitis pathogen reveals its unique biology.
bioRxiv : the preprint server for biology
O-GlcNAc modification of HSP27 alters its protein interactions and promotes refolding of proteins through the BAG3/HSP70 co-chaperone.
Protein science : a publication of the Protein Society
P66 is a bacterial mimic of CD47 that binds the anti-phagocytic receptor SIRPα and facilitates macrophage evasion by Borrelia burgdorferi.
bioRxiv : the preprint server for biology
Correction to "Changes in Metabolic Chemical Reporter Structure Yield a Selective Probe of O-GlcNAc Modification".
Journal of the American Chemical Society
Improved Electrophile Design for Exquisite Covalent Molecule Selectivity.
ACS chemical biology
Proteomic characterization of phagocytic primary human monocyte-derived macrophages.
RSC chemical biology
Refinement of Covalent EGFR Inhibitor AZD9291 to Eliminate Off-target Activity.
Tetrahedron letters
Metabolic Labeling for the Visualization and Identification of Potentially O-GlcNAc-Modified Proteins.
Current protocols in chemical biology
O-Acetylated Chemical Reporters of Glycosylation Can Display Metabolism-Dependent Background Labeling of Proteins but Are Generally Reliable Tools for the Identification of Glycoproteins.
Frontiers in chemistry
The Proteome-Wide Potential for Reversible Covalency at Cysteine.
Angewandte Chemie (International ed. in English)
Dimethyl Fumarate Disrupts Human Innate Immune Signaling by Targeting the IRAK4-MyD88 Complex.
Journal of immunology (Baltimore, Md. : 1950)
Azide- and Alkyne-Bearing Metabolic Chemical Reporters of Glycosylation Show Structure-Dependent Feedback Inhibition of the Hexosamine Biosynthetic Pathway.
Chembiochem : a European journal of chemical biology
Correction to "An Alkyne-Aspirin Chemical Reporter for the Detection of Aspirin-Dependent Protein Modification in Living Cells".
Journal of the American Chemical Society
The New Chemical Reporter 6-Alkynyl-6-deoxy-GlcNAc Reveals O-GlcNAc Modification of the Apoptotic Caspases That Can Block the Cleavage/Activation of Caspase-8.
Journal of the American Chemical Society
Metabolic Chemical Reporters of Glycans Exhibit Cell-Type-Selective Metabolism and Glycoprotein Labeling.
Chembiochem : a European journal of chemical biology
The Small Molecule 2-Azido-2-deoxy-glucose Is a Metabolic Chemical Reporter of O-GlcNAc Modifications in Mammalian Cells, Revealing an Unexpected Promiscuity of O-GlcNAc Transferase.
ACS chemical biology
Metabolically Labile Fumarate Esters Impart Kinetic Selectivity to Irreversible Inhibitors.
Journal of the American Chemical Society
Chemical proteomic map of dimethyl fumarate-sensitive cysteines in primary human T cells.
Science signaling
O-GlcNAc modification blocks the aggregation and toxicity of the protein α-synuclein associated with Parkinson's disease.
Nature chemistry
Changes in metabolic chemical reporter structure yield a selective probe of O-GlcNAc modification.
Journal of the American Chemical Society
Chemical reporter for visualizing metabolic cross-talk between carbohydrate metabolism and protein modification.
ACS chemical biology
An alkyne-aspirin chemical reporter for the detection of aspirin-dependent protein modification in living cells.
Journal of the American Chemical Society
Incorporation of unnatural sugars for the identification of glycoproteins.
Methods in molecular biology (Clifton, N.J.)
N-Propargyloxycarbamate monosaccharides as metabolic chemical reporters of carbohydrate salvage pathways and protein glycosylation.
Chemical communications (Cambridge, England)
Chemical reporters for fluorescent detection and identification of O-GlcNAc-modified proteins reveal glycosylation of the ubiquitin ligase NEDD4-1.
Proceedings of the National Academy of Sciences of the United States of America
Robust in-gel fluorescence detection of mucin-type O-linked glycosylation.
Bioorganic & medicinal chemistry letters
Precise control of protein concentration in living cells.
Angewandte Chemie (International ed. in English)
Address
555 & 535 Mission Bay Blvd South
San Francisco, CA 94158
United States