Professor
Cardiovascular Research Institute
Research Interests
Cell-cell signaling in development
Email
+1 415 476-4963
Faculty Type
Core CVRI Faculty
Research Summary:
Developmental regulation Summary: My laboratory investigates the mechanisms that pattern developing organs. We carry out our studies on the fruit fly, as it offers many advantages with its ready accessibility to histological analysis and the ease with which genetic manipulations can be made. We focus on two systems the fly wing and the fly lung. Both are model systems that offer opportunities to identify and characterize basic genetic and molecular mechanisms that are relevant to human development and disease.
Publications
Conserved roles of engrailed: patterning tissues and specifying cell types.
Development (Cambridge, England)
Metabolic control of progenitor cell propagation during Drosophila tracheal remodeling.
Nature communications
DaXi-high-resolution, large imaging volume and multi-view single-objective light-sheet microscopy.
Nature methods
Fly Cell Atlas: A single-nucleus transcriptomic atlas of the adult fruit fly.
Science (New York, N.Y.)
Preface.
Current topics in developmental biology
Elimination of nurse cell nuclei that shuttle into oocytes during oogenesis.
The Journal of Cell Biology
Cytonemes with complex geometries and composition extend into invaginations of target cells.
The Journal of Cell Biology
Hedgehog produced by the Drosophila wing imaginal disc induces distinct responses in three target tissues.
Development (Cambridge, England)
Broadly conserved roles of TMEM131 family proteins in intracellular collagen assembly and secretory cargo trafficking.
Science advances
Cytoneme-mediated signaling essential for tumorigenesis.
PLoS genetics
Designing a Green Fluorogenic Protease Reporter by Flipping a Beta Strand of GFP for Imaging Apoptosis in Animals.
Journal of the American Chemical Society
Glutamate signaling at cytoneme synapses.
Science (New York, N.Y.)
Withdrawal: Dm1-MMP, a matrix metalloproteinase from Drosophila with a potential role in extracellular matrix remodeling during neural development.
The Journal of biological chemistry
Visualizing Dynamics of Cell Signaling In Vivo with a Phase Separation-Based Kinase Reporter.
Molecular cell
Visualizing Dynamics of Cell Signaling In Vivo with a Phase Separation-Based Kinase Reporter.
Molecular cell
Scripting a place in time.
Developmental biology
Essential basal cytonemes take up Hedgehog in the Drosophila wing imaginal disc.
Development (Cambridge, England)
Hyperactive locomotion in a Drosophila model is a functional readout for the synaptic abnormalities underlying fragile X syndrome.
Science signaling
Macrophages Help Cells Connect to Pattern Zebrafish Stripes.
Developmental cell
Correction: Bicoid gradient formation and function in the Drosophila pre-syncytial blastoderm.
eLife
Distributing signaling proteins in space and time: the province of cytonemes.
Current opinion in genetics & development
Augmented noncanonical BMP type II receptor signaling mediates the synaptic abnormality of fragile X syndrome.
Science signaling
A Path to Pattern.
Current topics in developmental biology
Paracrine signaling mediated at cell-cell contacts.
BioEssays : news and reviews in molecular, cellular and developmental biology
Cytonemes and the dispersion of morphogens.
Wiley interdisciplinary reviews. Developmental biology
The contrasting roles of primary cilia and cytonemes in Hh signaling.
Developmental biology
Hedgehog and its circuitous journey from producing to target cells.
Seminars in cell & developmental biology
An improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging.
Nature communications
Communicating by touch--neurons are not alone.
Trends in cell biology
Cytonemes as specialized signaling filopodia.
Development (Cambridge, England)
Cytoneme-mediated contact-dependent transport of the Drosophila decapentaplegic signaling protein.
Science (New York, N.Y.)
Microarray comparison of anterior and posterior Drosophila wing imaginal disc cells identifies novel wing genes.
G3 (Bethesda, Md.)
Cytonemes extend their reach.
The EMBO journal
The imperatives of context and contour for morphogen dispersion.
Biophysical Journal
Barcoding Hedgehog for intracellular transport.
Science signaling
Direct delivery mechanisms of morphogen dispersion.
Science signaling
Specificity of Drosophila cytonemes for distinct signaling pathways.
Science (New York, N.Y.)
Hedgehog targets in the Drosophila embryo and the mechanisms that generate tissue-specific outputs of Hedgehog signaling.
Development (Cambridge, England)
Generalizing moving averages for tiling arrays using combined p-value statistics.
Statistical applications in genetics and molecular biology
The chromatin-remodeling protein Osa interacts with CyclinE in Drosophila eye imaginal discs.
Genetics
Regulation of Drosophila matrix metalloprotease Mmp2 is essential for wing imaginal disc:trachea association and air sac tubulogenesis.
Developmental biology
Two patched protein subtypes and a conserved domain of group I proteins that regulates turnover.
The Journal of biological chemistry
Organ renewal and cell divisions by differentiated cells in Drosophila.
Proceedings of the National Academy of Sciences of the United States of America
A screen for modifiers of hedgehog signaling in Drosophila melanogaster identifies swm and mts.
Genetics
Linear RNA amplification for the production of microarray hybridization probes.
Methods in molecular biology (Clifton, N.J.)
Understanding morphogen gradients: a problem of dispersion and containment.
Current opinion in genetics & development
The C-terminal tail of the Hedgehog receptor Patched regulates both localization and turnover.
Genes & development
Smoothened regulates activator and repressor functions of Hedgehog signaling via two distinct mechanisms.
The Journal of biological chemistry
Tracheal branch repopulation precedes induction of the Drosophila dorsal air sac primordium.
Developmental biology
Regulation of cellular plasticity in Drosophila imaginal disc cells by the Polycomb group, trithorax group and lama genes.
Development (Cambridge, England)
FGF is an essential mitogen and chemoattractant for the air sacs of the drosophila tracheal system.
Developmental cell
Expression profiling of Drosophila imaginal discs.
Genome biology
Another arrow in the Drosophila quiver.
Proceedings of the National Academy of Sciences of the United States of America
Dm1-MMP, a matrix metalloproteinase from Drosophila with a potential role in extracellular matrix remodeling during neural development.
The Journal of biological chemistry
Functional studies of the BTB domain in the Drosophila GAGA and Mod(mdg4) proteins.
Nucleic Acids Research
Expression of the vertebrate Gli proteins in Drosophila reveals a distribution of activator and repressor activities.
Development (Cambridge, England)
On the range of hedgehog signaling.
Current opinion in genetics & development
Hedgehog signal transduction in the posterior compartment of the Drosophila wing imaginal disc.
Molecular cell
The Drosophila genome sequence: implications for biology and medicine.
Science (New York, N.Y.)
GFP-tagged balancer chromosomes for Drosophila melanogaster.
Mechanisms of development
Ci: a complex transducer of the hedgehog signal.
Trends in genetics : TIG
GFP-tagged balancer chromosomes for Drosophila melanogaster.
Mechanisms of development
Pictures in cell biology. Cytonemes.
Trends in cell biology
Phosphorylation of the fused protein kinase in response to signaling from hedgehog.
Proceedings of the National Academy of Sciences of the United States of America
The Drosophila engrailed and invected genes: partners in regulation, expression and function.
Genetics
Creating a Drosophila wing de novo, the role of engrailed, and the compartment border hypothesis.
Development (Cambridge, England)
Selection and characterization of sequences with high affinity for the engrailed protein of Drosophila.
Mechanisms of development
Analysis of the genes involved in organizing the tail segments of the Drosophila melanogaster embryo.
Mechanisms of development
Analysis of cubitus interruptus regulation in Drosophila embryos and imaginal disks.
Development (Cambridge, England)
Expression and targeting of Syrian hamster prion protein induced by heat shock in transgenic Drosophila melanogaster.
Mechanisms of development
polyhomeotic appears to be a target of engrailed regulation in Drosophila.
Development (Cambridge, England)
Phosphorylation of the Drosophila engrailed protein at a site outside its homeodomain enhances DNA binding.
The Journal of biological chemistry
Allele-specific quantification of Drosophila engrailed and invected transcripts.
Proceedings of the National Academy of Sciences of the United States of America
Blistered: a gene required for vein/intervein formation in wings of Drosophila.
Development (Cambridge, England)
Understanding the homeodomain.
The Journal of biological chemistry
Isolation of cDNAs encoding the Drosophila GAGA transcription factor.
Molecular and Cellular Biology
DR-78, a novel Drosophila melanogaster genomic DNA fragment highly homologous to the DNA-binding domain of thyroid hormone-retinoic acid-vitamin D receptor subfamily.
Biochimica et biophysica acta
Segmentation of the Drosophila embryo.
Current opinion in genetics & development
The Drosophila hedgehog gene is expressed specifically in posterior compartment cells and is a target of engrailed regulation.
Genes & development
Development of the D. melanogaster caudal segments involves suppression of the ventral regions of A8, A9 and A10.
Development (Cambridge, England)
Expression during Drosophila development of DER, a gene related to erbB-1 and neu: correlations with mutant phenotypes.
Developmental biology
A gene related to the proto-oncogene fps/fes is expressed at diverse times during the life cycle of Drosophila melanogaster.
Molecular and Cellular Biology
MYB and MYC in the cell cycle.
Cold Spring Harbor symposia on quantitative biology
Diverse expression of dsrc29A, a gene related to src, during the life cycle of Drosophila melanogaster.
Development (Cambridge, England)
Region-specific recombination and expression are directed by portions of the Drosophila engrailed promoter.
Genes & development
Repression of ci-D in posterior compartments of Drosophila by engrailed.
Genes & development
Expression of engrailed during segmentation in grasshopper and crayfish.
Development (Cambridge, England)
fushi tarazu protein expression in the cellular blastoderm of Drosophila detected using a novel imaging technique.
Development (Cambridge, England)
Patterns of engrailed protein in early Drosophila embryos.
Development (Cambridge, England)
Association of the Drosophila melanogaster engrailed protein with specific soluble nuclear protein complexes.
The EMBO journal
Spatial regulation of engrailed expression in the Drosophila embryo.
Genes & development
The Drosophila engrailed protein is phosphorylated by a serine-specific protein kinase.
Nucleic Acids Research
In vitro transcription of the Drosophila engrailed gene.
Genes & development
Modifying expression of the engrailed gene of Drosophila melanogaster.
Development (Cambridge, England)
The transcription unit of the Drosophila engrailed locus: an unusually small portion of a 70,000 bp gene.
The EMBO journal
The invected gene of Drosophila: sequence analysis and expression studies reveal a close kinship to the engrailed gene.
Genes & development
Proto-oncogenes of Drosophila melanogaster.
Cold Spring Harbor symposia on quantitative biology
Structure and expression of two classes of mammalian homeo-box-containing genes.
Cold Spring Harbor symposia on quantitative biology
The engrailed locus of Drosophila melanogaster: genetic, developmental, and molecular studies.
Cold Spring Harbor symposia on quantitative biology
The phenotype of engrailed mutations in the antenna of Drosophila.
Developmental biology
Small nuclear RNA transcription and ribonucleoprotein assembly in early Xenopus development.
The Journal of Cell Biology
Three loci related to the src oncogene and tyrosine-specific protein kinase activity in Drosophila.
Nature
Compartments in the abdomen of Drosophila and the role of the engrailed locus.
Developmental biology
Engrailed: a gene controlling compartment and segment formation in Drosophila.
Proceedings of the National Academy of Sciences of the United States of America
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