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Engel

Joanne Engel, MD, PhD

Professor
Medicine
Address: 
513 Parnassus Avenue, HSE, Rm 405
UCSF Box 0654
San Francisco, CA 94143
United States
Phone: 
Email: 
[email protected]
UCSF Profiles

Research Interests: Bacterial Pathogen-Host Cell Interactions Summary: My laboratory is interested understanding and exploiting the complex interplay of microbial pathogens with eukaryotic cells. To that end, we have investigated the key processes of microbial attachment and entry, intracellular survival, and host cell injury in the context of two important human pathogens, Pseudomonas aeruginosa (PA) and Chlamydia trachomatis (CT). Each of these microorganisms has developed a unique strategy for successful survival that involves subverting and exploiting host cell pathways. Dissecting these processes will allow the development of new diagnostics, therapeutics, and vaccines and will provide a unique window into eukaryotic cell biology. 

Publications: 

The Chlamydia effector IncE employs two short linear motifs to reprogram host vesicle trafficking.

Cell reports

Pha K, Mirrashidi K, Sherry J, Tran CJ, Herrera CM, McMahon E, Elwell CA, Engel JN

Global mapping of the Chlamydia trachomatis conventional secreted effector - host interactome reveals CebN interacts with nucleoporins and Rae1 to impede STAT1 nuclear translocation.

bioRxiv : the preprint server for biology

Steiert B, Andersen SE, McCaslin PN, Elwell CA, Faris R, Tijerina X, Smith P, Eldridge Q, Imai BS, Arrington JV, Yau PM, Mirrashidi KM, Johnson JR, Verschueren E, Von Dollen J, Jang GM, Krogan NJ, Engel JN, Weber MM

The Chlamydia effector IncE employs two short linear motifs to reprogram host vesicle trafficking.

bioRxiv : the preprint server for biology

Pha K, Mirrashidi K, Sherry J, Tran CJ, Herrera CM, McMahon E, Elwell CA, Engel JN

Spatial control of sensory adaptation modulates mechanosensing in Pseudomonas aeruginosa.

bioRxiv : the preprint server for biology

Patino R, Kühn MJ, Macmillan H, Inclan YF, Chavez I, Von Dollen J, Johnson JR, Swaney DL, Krogan NJ, Persat A, Engel JN

Peptidoglycan-Targeted [18F]3,3,3-Trifluoro-d-alanine Tracer for Imaging Bacterial Infection.

JACS Au

Alexandre M. Sorlin, Marina Lo´pez-A´lvarez, Jacob Biboy, Joe Gray, Sarah J. Rabbitt, Junaid Ur Rahim, Sang Hee Lee, Kondapa Naidu Bobba, Joseph Blecha, Mathew F.L. Parker, Robert R. Flavell, Joanne Engel, Michael Ohliger, Waldemar Vollmer, David M. Wilson

Two antagonistic response regulators control Pseudomonas aeruginosa polarization during mechanotaxis.

The EMBO journal

Kühn MJ, Macmillan H, Talà L, Inclan Y, Patino R, Pierrat X, Al-Mayyah Z, Engel JN, Persat A

Imaging joint infections using D-methyl-11C-methionine PET/MRI: initial experience in humans.

European journal of nuclear medicine and molecular imaging

Polvoy I, Seo Y, Parker M, Stewart M, Siddiqua K, Manacsa HS, Ravanfar V, Blecha J, Hope TA, Vanbrocklin H, Flavell RR, Barry J, Hansen E, Villanueva-Meyer JE, Engel J, Rosenberg OS, Wilson DM, Ohliger MA

Mechanotaxis directs Pseudomonas aeruginosa twitching motility.

Proceedings of the National Academy of Sciences of the United States of America

Kühn MJ, Talà L, Inclan YF, Patino R, Pierrat X, Vos I, Al-Mayyah Z, Macmillan H, Negrete J, Engel JN, Persat A

Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi.

Nature microbiology

Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu J, Inclan YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS

Emerging Role of Retromer in Modulating Pathogen Growth.

Trends in microbiology

Elwell C, Engel J

Targeting the Mucosal Barrier: How Pathogens Modulate the Cellular Polarity Network.

Cold Spring Harbor perspectives in biology

Ruch TR, Engel JN

Chlamydia interfere with an interaction between the mannose-6-phosphate receptor and sorting nexins to counteract host restriction.

eLife

Elwell CA, Czudnochowski N, von Dollen J, Johnson JR, Nakagawa R, Mirrashidi K, Krogan NJ, Engel JN, Rosenberg OS

Par3 integrates Tiam1 and phosphatidylinositol 3-kinase signaling to change apical membrane identity.

Molecular biology of the cell

Ruch TR, Bryant DM, Mostov KE, Engel JN

A scaffold protein connects type IV pili with the Chp chemosensory system to mediate activation of virulence signaling in Pseudomonas aeruginosa.

Molecular microbiology

Inclan YF, Persat A, Greninger A, Von Dollen J, Johnson J, Krogan N, Gitai Z, Engel JN

Chlamydia cell biology and pathogenesis.

Nature reviews. Microbiology

Elwell C, Mirrashidi K, Engel J

Global Mapping of the Inc-Human Interactome Reveals that Retromer Restricts Chlamydia Infection.

Cell host & microbe

Mirrashidi KM, Elwell CA, Verschueren E, Johnson JR, Frando A, Von Dollen J, Rosenberg O, Gulbahce N, Jang G, Johnson T, Jäger S, Gopalakrishnan AM, Sherry J, Dunn JD, Olive A, Penn B, Shales M, Cox JS, Starnbach MN, Derre I, Valdivia R, Krogan NJ, Engel J

Type IV pili mechanochemically regulate virulence factors in Pseudomonas aeruginosa.

Proceedings of the National Academy of Sciences of the United States of America

Persat A, Inclan YF, Engel JN, Stone HA, Gitai Z

The Pseudomonas aeruginosa type III translocon is required for biofilm formation at the epithelial barrier.

PLoS pathogens

Tran CS, Rangel SM, Almblad H, Kierbel A, Givskov M, Tolker-Nielsen T, Hauser AR, Engel JN

Host cell polarity proteins participate in innate immunity to Pseudomonas aeruginosa infection.

Cell host & microbe

Tran CS, Eran Y, Ruch TR, Bryant DM, Datta A, Brakeman P, Kierbel A, Wittmann T, Metzger RJ, Mostov KE, Engel JN

Cystic fibrosis epithelial cell and bacterial binding.

American journal of physiology. Lung cellular and molecular physiology

Engel J, Matthay M

Sugar administration is an effective adjunctive therapy in the treatment of Pseudomonas aeruginosa pneumonia.

American journal of physiology. Lung cellular and molecular physiology

Bucior I, Abbott J, Song Y, Matthay MA, Engel JN

Chlamydial intracellular survival strategies.

Cold Spring Harbor perspectives in medicine

Bastidas RJ, Elwell CA, Engel JN, Valdivia RH

Endosulfatases SULF1 and SULF2 limit Chlamydia muridarum infection.

Cellular microbiology

Kim JH, Chan C, Elwell C, Singer MS, Dierks T, Lemjabbar-Alaoui H, Rosen SD, Engel JN

Lipid acquisition by intracellular Chlamydiae.

Cellular microbiology

Elwell CA, Engel JN

Chlamydia trachomatis co-opts the FGF2 signaling pathway to enhance infection.

PLoS pathogens

Kim JH, Jiang S, Elwell CA, Engel JN

Chlamydia trachomatis co-opts GBF1 and CERT to acquire host sphingomyelin for distinct roles during intracellular development.

PLoS pathogens

Elwell CA, Jiang S, Kim JH, Lee A, Wittmann T, Hanada K, Melancon P, Engel JN

Cell migration regulates the kinetics of cytokinesis.

Cell cycle (Georgetown, Tex.)

Wood S, Sivaramakrishnan G, Engel J, Shafikhani SH

FimL regulates cAMP synthesis in Pseudomonas aeruginosa.

PloS one

Inclan YF, Huseby MJ, Engel JN

Role of Pseudomonas aeruginosa type III effectors in disease.

Current opinion in microbiology

Engel J, Balachandran P

Focal adhesion components are essential for mammalian cell cytokinesis.

Cell cycle (Georgetown, Tex.)

Shafikhani SH, Mostov K, Engel J

Roles of type IV pili, flagellum-mediated motility and extracellular DNA in the formation of mature multicellular structures in Pseudomonas aeruginosa biofilms.

Environmental microbiology

Barken KB, Pamp SJ, Yang L, Gjermansen M, Bertrand JJ, Klausen M, Givskov M, Whitchurch CB, Engel JN, Tolker-Nielsen T

Use of a novel coinfection system reveals a role for Rac1, H-Ras, and CrkII phosphorylation in Helicobacter pylori-induced host cell actin cytoskeletal rearrangements.

FEMS immunology and medical microbiology

Brandt S, Shafikhani S, Balachandran P, Jin S, Hartig R, König W, Engel J, Backert S

Pseudomonas aeruginosa exploits a PIP3-dependent pathway to transform apical into basolateral membrane.

The Journal of Cell Biology

Kierbel A, Gassama-Diagne A, Rocha C, Radoshevich L, Olson J, Mostov K, Engel J

Copper and nitric oxide meet in the plasma.

Nature chemical biology

Samuel TK, Gitlin JD

An essential, putative MEK kinase of Leishmania major.

Molecular and biochemical parasitology

Agron PG, Reed SL, Engel JN

Pseudomonas aeruginosa fimL regulates multiple virulence functions by intersecting with Vfr-modulated pathways.

Molecular microbiology

Whitchurch CB, Beatson SA, Comolli JC, Jakobsen T, Sargent JL, Bertrand JJ, West J, Klausen M, Waite LL, Kang PJ, Tolker-Nielsen T, Mattick JS, Engel JN

Lipid raft-mediated entry is not required for Chlamydia trachomatis infection of cultured epithelial cells.

Infection and immunity

Gabel BR, Elwell C, van Ijzendoorn SC, Engel JN

A mycobacterial virulence gene cluster extending RD1 is required for cytolysis, bacterial spreading and ESAT-6 secretion.

Molecular microbiology

Gao LY, Guo S, McLaughlin B, Morisaki H, Engel JN, Brown EJ

Tarp and Arp: How Chlamydia induces its own entry.

Proceedings of the National Academy of Sciences of the United States of America

Engel J

Characterization of a complex chemosensory signal transduction system which controls twitching motility in Pseudomonas aeruginosa.

Molecular microbiology

Whitchurch CB, Leech AJ, Young MD, Kennedy D, Sargent JL, Bertrand JJ, Semmler AB, Mellick AS, Martin PR, Alm RA, Hobbs M, Beatson SA, Huang B, Nguyen L, Commolli JC, Engel JN, Darzins A, Mattick JS

The ADP ribosyltransferase domain of Pseudomonas aeruginosa ExoT contributes to its biological activities.

Infection and immunity

Garrity-Ryan L, Shafikhani S, Balachandran P, Nguyen L, Oza J, Jakobsen T, Sargent J, Fang X, Cordwell S, Matthay MA, Engel JN

Epithelial cell polarity alters Rho-GTPase responses to Pseudomonas aeruginosa.

Molecular biology of the cell

Kazmierczak BI, Mostov K, Engel JN

Molecular Pathogenesis of Acute Pseudomonas Aeruginosa Infections.

Severe Infections Caused by Pseudomonas Aeruginosa

Joanne N. Engel

Pseudomonas aeruginosa ExoT inhibits in vitro lung epithelial wound repair.

Cellular microbiology

Geiser TK, Kazmierczak BI, Garrity-Ryan LK, Matthay MA, Engel JN

Rho GTPase activity modulates Pseudomonas aeruginosa internalization by epithelial cells.

Cellular microbiology

Kazmierczak BI, Jou TS, Mostov K, Engel JN

Interaction of bacterial pathogens with polarized epithelium.

Annual review of microbiology

Kazmierczak BI, Mostov K, Engel JN

Host cell-derived sphingolipids are required for the intracellular growth of Chlamydia trachomatis.

Cellular microbiology

van Ooij C, Kalman L, Nishijima M, Hanada K, Mostov K, Engel JN

Eukaryotic cell uptake of heparin-coated microspheres: a model of host cell invasion by Chlamydia trachomatis.

Infection and immunity

Stephens RS, Fawaz FS, Kennedy KA, Koshiyama K, Nichols B, van Ooij C, Engel JN

Defects in type III secretion correlate with internalization of Pseudomonas aeruginosa by epithelial cells.

Infection and immunity

Hauser AR, Fleiszig S, Kang PJ, Mostov K, Engel JN

Identification of Pseudomonas aeruginosa genes required for epithelial cell injury.

Molecular microbiology

Kang PJ, Hauser AR, Apodaca G, Fleiszig SM, Wiener-Kronish J, Mostov K, Engel JN

Characterization of Pseudomonas aeruginosa-induced MDCK cell injury: glycosylation-defective host cells are resistant to bacterial killing.

Infection and immunity

Apodaca G, Bomsel M, Lindstedt R, Engel J, Frank D, Mostov KE, Wiener-Kronish J

Stage-specific regulation of protein phosphorylation in Leishmania major.

Molecular and biochemical parasitology

Dell KR, Engel JN

Cloning and characterization of the RNA polymerase alpha-subunit operon of Chlamydia trachomatis.

Journal of bacteriology

Tan M, Klein R, Grant R, Ganem D, Engel J

A developmentally regulated chlamydial gene with apparent homology to eukaryotic histone H1.

Proceedings of the National Academy of Sciences of the United States of America

Perara E, Ganem D, Engel JN

Heat shock response of murine Chlamydia trachomatis.

Journal of bacteriology

Engel JN, Pollack J, Perara E, Ganem D

21 Identification and Comparison of Putative Chlamydial Promoter Elements.

Immune Recognition and Evasion: Molecular Aspects of Host?parasite Interaction

JOANNE N. ENGEL, DON GANEM

Phenytoin hypersensitivity: a case of severe acute rhabdomyolysis.

The American journal of medicine

Engel JN, Mellul VG, Goodman DB

Phenytoin hypersensitivity: a case of severe acute rhabdomyolysis

American Journal of Medicine

Engel, JN, Mellul, VG, Goodman, DBP

Evolutionary conservation in the untranslated regions of actin mRNA's

Nucleic Acids Research

Ponte, P, Ng, SY, Engel, JN, Kedes, LH

Isolation and characterization of human actin genes. Ph.D. Dissertation, Stanford University

Isolation and characterization of human actin genes. Ph.D. Dissertation, Stanford University

Engel, JN

Molecular genetics of human myogenesis

Experimental Biology and Medicine

Kedes, LH, Blau, H, Gunning, P, Ponte, P, Chiu, C-P, Bain, W, Engel, JN, Guttman, S

Human cytoplasmic actin proteins are encoded by a multigene family

Molecular and Cellular Biology

Engel, JN, Gunning, P, Kedes, LH

Isolation and characterization of human actin genes.

Proceedings of the National Academy of Sciences of the United States of America

Engel JN, Gunning PW, Kedes L

Isolation and characterization of human actin genes

Proceedings National Academy of Sciences

Engel, JN, Gunning, P, Kedes, LH

Faculty Type: 
Affiliate Faculty