Home > People > Keith Mostov, MD, PhD
Mostov

Keith Mostov, MD, PhD

Professor
Anatomy
Address: 
600 16th Street, Rm N212B
UCSF Box 2140
San Francisco, CA 94158
United States
Phone: 
+1 415 606-6308

Research Interests: Polarized epithelial membrane traffic and epithelial morphogenesis. Summary: How do individual cells organize to form a multicellular tissue? An individual cell can exhibit many different behaviors - proliferation, migration, adhesion, polarization, differentiation, and death. But to build a tissue, a population of cells must coordinate these individual behaviors across space and time. Little is understood about the mechanisms that orchestrate the actions of single cells during morphogenesis. To analyze these issues, we are studying how epithelial cells form three-dimensional organs. Epithelia are coherent sheets of cells that form a barrier between the interior of the body and the outside world. Internal epithelial organs contain two types of building blocks, cysts and tubules. Our experimental strategy uses culture of epithelial cells in a three-dimensional extracellular matrix. Single cells plated in matrix grow to form hollow cysts lined by a monolayer of cells. We have discovered a pathway containing the small GTPase, rac1, alpha1-beta3 integrin, and laminin, which coordinates cell polarity, so that apical surfaces of the cells are all oriented towards the cyst lumen. Cysts are remodeled into by growth factors, which cause transient dedifferentiation and migration, followed by redifferentiation into polarized epithelial cells lining the tubule. Spatial asymmetry is fundamental to the structure and function of most eukaryotic cells. A basic aspect of this polarity is that the cell's plasma membrane is divided into discrete domains. The best studied and simplest example of this occurs in epithelial cells, which line exposed body surfaces. Epithelial cells have an apical surface facing the outside world and a basolateral surface contacting adjacent cells and the underlying connective tissue. These surfaces have completely different compositions. Epithelial cells use two pathways to send proteins to the cell surface. Newly made proteins can travel directly from the trans-Golgi network (TGN) to either the apical or basolateral surface. Alternatively, proteins can be sent to the basolateral surface and then endocytosed and transcytosed to the apical surface. We are studying the machinery that is responsible for the specificity and regulation of polarized membrane traffic in epithelial cells. I will discuss several recent results. 1. The SNARE hypothesis provides a unified model for how intracellular vesicular targeting and fusion work. Proteins on transport vesicles, known as v-SNAREs, pair with corresponding t-SNAREs on target membranes, leading to vesicle fusion. The correct pairing of particular v- and t-SNAREs can provide a mechanism for specificity of targeting and fusion. Polarized epithelial cells are an ideal system in which to test the role of SNAREs in specificity, as these cells contain two plasma membrane targets, the apical and basolateral surfaces, as well as multiple classes of vesicles traveling to each surface. We have found that that the t-SNARE syntaxin 3, is involved with transport to the apical surface, while the related t-SNARE, syntaxin 4, is utilized for transport to the basolateral surface. 2. The polymeric immunoglobulin receptor (pIgR) transcytoses IgA from the basolateral to the apical surface. Transcytosis is stimulated by ligand binding. Binding of IgA causes dimerization of the pIgR, which leads to activation of a non-receptor tyrosine kinase, p62Yes. Mice knocked out for this kinase are deficient in IgA transport. Phosphatidylinositol-specific phospholipase C gamma is activated, resulting in production of DAG and IP3. The DAG activates protein kinase Ce, which stimulates transcytosis. The IP3 raises intracellular free calcium, which also stimulates transcytosis. Stimulation of transcytosis also involves the small GTPase, rab3b, which directly interacts with the pIgR. 3. When epithelial cells, such as MDCK cells, are plated in a 3 dimensional collagen matrix, the cells form hollow, polarized cysts with the apical surface facing the lumen of the cyst. Overexpression of a dominant negative form of the small GTPase, rac, retards lumen formation and leads to a partial reversal of polarity, with the apical surface oriented towards the outside of the cyst. Growth of the cysts laminin rescues this phenotype, indicating that interfering with rac function interferes with the ability of the cell to assemble, laminin, which normally provides a spatial cue. 4. When collagen-grown cysts are stimulated with hepatocyte growth factor (HGF), the cysts develop branching tubules, providing a simple model system for studying tubulogenesis. The exocyst is an eight-subunit complex involved in targeting transport vesicles to specific regions of the plasma membrane. We have found that HGF treatment causes the exocyst to relocalize from the region of the tight junction to the growing tubule, indicating that new membrane is being directed to the tubule. Overexpression a subunit of the exocyst, hSec10, causes the cysts to elaborate an increased umber of tubules, indicating a direct connection between membrane traffic and tubulogenesis.

Publications: 

Vascular and Liver Homeostasis in Juvenile Mice Require Endothelial Cyclic AMP-Dependent Protein Kinase A.

International journal of molecular sciences

Nedvetsky PI, Cornelissen I, Mathivet T, Bouleti C, Ou P, Baatsen P, Zhao X, Schuit F, Stanchi F, Mostov KE, Gerhardt H

Ciliary Hedgehog signaling patterns the digestive system to generate mechanical forces driving elongation.

Nature communications

Yang Y, Paivinen P, Xie C, Krup AL, Makela TP, Mostov KE, Reiter JF

Author Correction: Guidelines and definitions for research on epithelial-mesenchymal transition.

Nature reviews. Molecular cell biology

Yang J, Antin P, Berx G, Blanpain C, Brabletz T, Bronner M, Campbell K, Cano A, Casanova J, Christofori G, Dedhar S, Derynck R, Ford HL, Fuxe J, García de Herreros A, Goodall GJ, Hadjantonakis AK, Huang RYJ, Kalcheim C, Kalluri R, Kang Y, Khew-Goodall Y, Levine H, Liu J, Longmore GD, Mani SA, Massagué J, Mayor R, McClay D, Mostov KE, Newgreen DF, Nieto MA, Puisieux A, Runyan R, Savagner P, Stanger B, Stemmler MP, Takahashi Y, Takeichi M, Theveneau E, Thiery JP, Thompson EW, Weinberg RA, Williams ED, Xing J, Zhou BP, Sheng G, EMT International Association (TEMTIA)

Intussusceptive Angiogenesis in Human Metastatic Malignant Melanoma.

The American journal of pathology

Pandita A, Ekstrand M, Bjursten S, Zhao Z, Fogelstrand P, Le Gal K, Ny L, Bergo MO, Karlsson J, Nilsson JA, Akyürek LM, Levin MC, Borén J, Ewald AJ, Mostov KE, Levin M

Guidelines and definitions for research on epithelial-mesenchymal transition.

Nature reviews. Molecular cell biology

Yang J, Antin P, Berx G, Blanpain C, Brabletz T, Bronner M, Campbell K, Cano A, Casanova J, Christofori G, Dedhar S, Derynck R, Ford HL, Fuxe J, García de Herreros A, Goodall GJ, Hadjantonakis AK, Huang RYJ, Kalcheim C, Kalluri R, Kang Y, Khew-Goodall Y, Levine H, Liu J, Longmore GD, Mani SA, Massagué J, Mayor R, McClay D, Mostov KE, Newgreen DF, Nieto MA, Puisieux A, Runyan R, Savagner P, Stanger B, Stemmler MP, Takahashi Y, Takeichi M, Theveneau E, Thiery JP, Thompson EW, Weinberg RA, Williams ED, Xing J, Zhou BP, Sheng G, EMT International Association (TEMTIA)

Simple Rules Determine Distinct Patterns of Branching Morphogenesis.

Cell systems

Yu W, Marshall WF, Metzger RJ, Brakeman PR, Morsut L, Lim W, Mostov KE

The phospholipid PI(3,4)P2 is an apical identity determinant.

Nature communications

Román-Fernández Á, Roignot J, Sandilands E, Nacke M, Mansour MA, McGarry L, Shanks E, Mostov KE, Bryant DM

Adaptor protein CD2AP and L-type lectin LMAN2 regulate exosome cargo protein trafficking through the Golgi complex.

The Journal of biological chemistry

Kwon SH, Oh S, Nacke M, Mostov KE, Lipschutz JH

Afadin orients cell division to position the tubule lumen in developing renal tubules.

Development (Cambridge, England)

Gao L, Yang Z, Hiremath C, Zimmerman SE, Long B, Brakeman PR, Mostov KE, Bryant DM, Luby-Phelps K, Marciano DK

Par3 integrates Tiam1 and phosphatidylinositol 3-kinase signaling to change apical membrane identity.

Molecular biology of the cell

Ruch TR, Bryant DM, Mostov KE, Engel JN

Adaptor Protein CD2AP and L-type Lectin LMAN2 Regulate Exosome Cargo Protein Trafficking through the Golgi Complex.

The Journal of biological chemistry

Kwon SH, Oh S, Nacke M, Mostov KE, Lipschutz JH

cAMP-dependent protein kinase A (PKA) regulates angiogenesis by modulating tip cell behavior in a Notch-independent manner.

Development (Cambridge, England)

Nedvetsky PI, Zhao X, Mathivet T, Aspalter IM, Stanchi F, Metzger RJ, Mostov KE, Gerhardt H

p114RhoGEF governs cell motility and lumen formation during tubulogenesis through a ROCK-myosin-II pathway.

Journal of cell science

Kim M, M Shewan A, Ewald AJ, Werb Z, Mostov KE

Phosphoinositide 3-kinase p110δ promotes lumen formation through the enhancement of apico-basal polarity and basal membrane organization.

Nature communications

Peng J, Awad A, Sar S, Komaiha OH, Moyano R, Rayal A, Samuel D, Shewan A, Vanhaesebroeck B, Mostov K, Gassama-Diagne A

A molecular switch for the orientation of epithelial cell polarization.

Developmental cell

Bryant DM, Roignot J, Datta A, Overeem AW, Kim M, Yu W, Peng X, Eastburn DJ, Ewald AJ, Werb Z, Mostov KE

Parasympathetic innervation regulates tubulogenesis in the developing salivary gland.

Developmental cell

Nedvetsky PI, Emmerson E, Finley JK, Ettinger A, Cruz-Pacheco N, Prochazka J, Haddox CL, Northrup E, Hodges C, Mostov KE, Hoffman MP, Knox SM

Host cell polarity proteins participate in innate immunity to Pseudomonas aeruginosa infection.

Cell host & microbe

Tran CS, Eran Y, Ruch TR, Bryant DM, Datta A, Brakeman P, Kierbel A, Wittmann T, Metzger RJ, Mostov KE, Engel JN

Polarity, cell division, and out-of-equilibrium dynamics control the growth of epithelial structures.

The Journal of Cell Biology

Cerruti B, Puliafito A, Shewan AM, Yu W, Combes AN, Little MH, Chianale F, Primo L, Serini G, Mostov KE, Celani A, Gamba A

Apical targeting of the formin Diaphanous in Drosophila tubular epithelia.

eLife

Rousso T, Shewan AM, Mostov KE, Schejter ED, Shilo BZ

Polarity in mammalian epithelial morphogenesis.

Cold Spring Harbor perspectives in biology

Roignot J, Peng X, Mostov K

Cell height: Tao rising.

The Journal of Cell Biology

Cai L, Mostov KE

Synaptotagmin-like proteins control the formation of a single apical membrane domain in epithelial cells.

Nature cell biology

Gálvez-Santisteban M, Rodriguez-Fraticelli AE, Bryant DM, Vergarajauregui S, Yasuda T, Bañón-Rodríguez I, Bernascone I, Datta A, Spivak N, Young K, Slim CL, Brakeman PR, Fukuda M, Mostov KE, Martín-Belmonte F

Cyclic AMP regulates formation of mammary epithelial acini in vitro.

Molecular biology of the cell

Nedvetsky PI, Kwon SH, Debnath J, Mostov KE

Scrib regulates HGF-mediated epithelial morphogenesis and is stabilized by Sgt1-HSP90.

Journal of cell science

Eastburn DJ, Zegers MM, Mostov KE

Reduced immunoglobulin A transcytosis associated with immunoglobulin A nephropathy and nasopharyngeal carcinoma.

The Journal of biological chemistry

Su T, Chapin SJ, Bryant DM, Shewan AM, Young K, Mostov KE

Regulation of intrahepatic biliary duct morphogenesis by Claudin 15-like b.

Developmental biology

Cheung ID, Bagnat M, Ma TP, Datta A, Evason K, Moore JC, Lawson ND, Mostov KE, Moens CB, Stainier DY

p120 catenin is required for normal renal tubulogenesis and glomerulogenesis.

Development (Cambridge, England)

Marciano DK, Brakeman PR, Lee CZ, Spivak N, Eastburn DJ, Bryant DM, Beaudoin GM, Hofmann I, Mostov KE, Reichardt LF

MDCK cystogenesis driven by cell stabilization within computational analogues.

PLoS computational biology

Engelberg JA, Datta A, Mostov KE, Hunt CA

Molecular regulation of lumen morphogenesis.

Current biology : CB

Datta A, Bryant DM, Mostov KE

Rab GTPase-Myo5B complexes control membrane recycling and epithelial polarization.

Proceedings of the National Academy of Sciences of the United States of America

Roland JT, Bryant DM, Datta A, Itzen A, Mostov KE, Goldenring JR

Transcriptional profiling identifies TNS4 function in epithelial tubulogenesis.

Current biology : CB

Kwon SH, Nedvetsky PI, Mostov KE

A kinase cascade leading to Rab11-FIP5 controls transcytosis of the polymeric immunoglobulin receptor.

Nature cell biology

Su T, Bryant DM, Luton F, Vergés M, Ulrich SM, Hansen KC, Datta A, Eastburn DJ, Burlingame AL, Shokat KM, Mostov KE

Cse1l is a negative regulator of CFTR-dependent fluid secretion.

Current biology : CB

Bagnat M, Navis A, Herbstreith S, Brand-Arzamendi K, Curado S, Gabriel S, Mostov K, Huisken J, Stainier DY

A molecular network for de novo generation of the apical surface and lumen.

Nature cell biology

Bryant DM, Datta A, Rodríguez-Fraticelli AE, Peränen J, Martín-Belmonte F, Mostov KE

STAT1 is required for redifferentiation during Madin-Darby canine kidney tubulogenesis.

Molecular biology of the cell

Kim M, O'Brien LE, Kwon SH, Mostov KE

The Cdc42 GEF Intersectin 2 controls mitotic spindle orientation to form the lumen during epithelial morphogenesis.

The Journal of Cell Biology

Rodriguez-Fraticelli AE, Vergarajauregui S, Eastburn DJ, Datta A, Alonso MA, Mostov K, Martín-Belmonte F

Simulation of lung alveolar epithelial wound healing in vitro.

Journal of the Royal Society, Interface

Kim SH, Matthay MA, Mostov K, Hunt CA

Polarity is destiny.

Cell

Cai L, Mostov K

Computational investigation of epithelial cell dynamic phenotype in vitro.

Theoretical biology & medical modelling

Kim SH, Park S, Mostov K, Debnath J, Hunt CA

Identification of a cytoplasmic signal for apical transcytosis.

Traffic (Copenhagen, Denmark)

Luton F, Hexham MJ, Zhang M, Mostov KE

A computational approach to understand in vitro alveolar morphogenesis.

PloS one

Kim SH, Yu W, Mostov K, Matthay MA, Hunt CA

Nectin proteins are expressed at early stages of nephrogenesis and play a role in renal epithelial cell morphogenesis.

American journal of physiology. Renal physiology

Brakeman PR, Liu KD, Shimizu K, Takai Y, Mostov KE

From cells to organs: building polarized tissue.

Nature reviews. Molecular cell biology

Bryant DM, Mostov KE

Focal adhesion components are essential for mammalian cell cytokinesis.

Cell cycle (Georgetown, Tex.)

Shafikhani SH, Mostov K, Engel J

Involvement of RhoA, ROCK I and myosin II in inverted orientation of epithelial polarity.

EMBO reports

Yu W, Shewan AM, Brakeman P, Eastburn DJ, Datta A, Bryant DM, Fan QW, Weiss WA, Zegers MM, Mostov KE

Cell-polarity dynamics controls the mechanism of lumen formation in epithelial morphogenesis.

Current biology : CB

Martín-Belmonte F, Yu W, Rodríguez-Fraticelli AE, Ewald AJ, Ewald A, Werb Z, Alonso MA, Mostov K

Regulation of cell polarity during epithelial morphogenesis.

Current opinion in cell biology

Martin-Belmonte F, Mostov K

In silico simulation of epithelial cell tubulogenesis.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference

Engelberg JA, Kim M, Mostov KE, Hunt CA

Development: inflationary pressures.

Nature

Bryant D, Mostov K

Catch the KIF5B train to the apical surface.

Developmental cell

Jacobson C, Mostov K

Rac1 is required for reorientation of polarity and lumen formation through a PI 3-kinase-dependent pathway.

American journal of physiology. Renal physiology

Liu KD, Datta A, Yu W, Brakeman PR, Jou TS, Matthay MA, Mostov KE

Genetic control of single lumen formation in the zebrafish gut.

Nature cell biology

Bagnat M, Cheung ID, Mostov KE, Stainier DY

Polarity proteins PAR6 and aPKC regulate cell death through GSK-3beta in 3D epithelial morphogenesis.

Journal of cell science

Kim M, Datta A, Brakeman P, Yu W, Mostov KE

Disruption of apical-basal polarity of human embryonic stem cells enhances hematoendothelial differentiation.

Stem cells (Dayton, Ohio)

Krtolica A, Genbacev O, Escobedo C, Zdravkovic T, Nordstrom A, Vabuena D, Nath A, Simon C, Mostov K, Fisher SJ

Phosphoinositides control epithelial development.

Cell cycle (Georgetown, Tex.)

Martin-Belmonte F, Mostov K

EGF induces macropinocytosis and SNX1-modulated recycling of E-cadherin.

Journal of cell science

Bryant DM, Kerr MC, Hammond LA, Joseph SR, Mostov KE, Teasdale RD, Stow JL

Pseudomonas aeruginosa exploits a PIP3-dependent pathway to transform apical into basolateral membrane.

The Journal of Cell Biology

Kierbel A, Gassama-Diagne A, Rocha C, Radoshevich L, Olson J, Mostov K, Engel J

Formation of cysts by alveolar type II cells in three-dimensional culture reveals a novel mechanism for epithelial morphogenesis.

Molecular biology of the cell

Yu W, Fang X, Ewald A, Wong K, Hunt CA, Werb Z, Matthay MA, Mostov K

PTEN-mediated apical segregation of phosphoinositides controls epithelial morphogenesis through Cdc42.

Cell

Martin-Belmonte F, Gassama A, Datta A, Yu W, Rescher U, Gerke V, Mostov K

A model of intussusceptive angiogenesis.

Novartis Foundation symposium

Levin M, Ewald AJ, McMahon M, Werb Z, Mostov K

Vesicle transport, cilium formation, and membrane specialization: the origins of a sensory organelle.

Proceedings of the National Academy of Sciences of the United States of America

Reiter JF, Mostov K

Phosphatidylinositol-3,4,5-trisphosphate regulates the formation of the basolateral plasma membrane in epithelial cells.

Nature cell biology

Gassama-Diagne A, Yu W, ter Beest M, Martin-Belmonte F, Kierbel A, Engel J, Mostov K

Simulating properties of in vitro epithelial cell morphogenesis.

PLoS computational biology

Grant MR, Mostov KE, Tlsty TD, Hunt CA

Developmental biology: the hole picture.

Nature

Mostov K, Martin-Belmonte F

A dual PI3 kinase/mTOR inhibitor reveals emergent efficacy in glioma.

Cancer cell

Fan QW, Knight ZA, Goldenberg DD, Yu W, Mostov KE, Stokoe D, Shokat KM, Weiss WA

mTOR is out of control in polycystic kidney disease.

Proceedings of the National Academy of Sciences of the United States of America

Mostov KE

Morphological and biochemical analysis of Rac1 in three-dimensional epithelial cell cultures.

Methods in enzymology

O'Brien LE, Yu W, Tang K, Jou TS, Zegers MM, Mostov KE

The role of syntaxins in the specificity of vesicle targeting in polarized epithelial cells.

Molecular biology of the cell

ter Beest MB, Chapin SJ, Avrahami D, Mostov KE

Formation of multicellular epithelial structures.

Novartis Foundation symposium

Mostov K, Brakeman P, Datta A, Gassama A, Katz L, Kim M, Leroy P, Levin M, Liu K, Martin F, O'Brien LE, Verges M, Su T, Tang K, Tanimizu N, Yamaji T, Yu W

Beta1-integrin orients epithelial polarity via Rac1 and laminin.

Molecular biology of the cell

Yu W, Datta A, Leroy P, O'Brien LE, Mak G, Jou TS, Matlin KS, Mostov KE, Zegers MM

Long-term culture of hepatic progenitors derived from mouse Dlk+ hepatoblasts.

Journal of cell science

Tanimizu N, Saito H, Mostov K, Miyajima A

The mammalian retromer regulates transcytosis of the polymeric immunoglobulin receptor.

Nature cell biology

Vergés M, Luton F, Gruber C, Tiemann F, Reinders LG, Huang L, Burlingame AL, Haft CR, Mostov KE

ERK and MMPs sequentially regulate distinct stages of epithelial tubule development.

Developmental cell

O'Brien LE, Tang K, Kats ES, Schutz-Geschwender A, Lipschutz JH, Mostov KE

Caspase induction by IgA antimitochondrial antibody: IgA-mediated biliary injury in primary biliary cirrhosis.

Hepatology (Baltimore, Md.)

Matsumura S, Van De Water J, Leung P, Odin JA, Yamamoto K, Gores GJ, Mostov K, Ansari AA, Coppel RL, Shiratori Y, Gershwin ME

Epithelial cell polarity alters Rho-GTPase responses to Pseudomonas aeruginosa.

Molecular biology of the cell

Kazmierczak BI, Mostov K, Engel JN

Hepatocyte growth factor induces MDCK cell morphogenesis without causing loss of tight junction functional integrity.

American journal of physiology. Cell physiology

Pollack AL, Apodaca G, Mostov KE

Pak1 and PIX regulate contact inhibition during epithelial wound healing.

The EMBO journal

Zegers MM, Forget MA, Chernoff J, Mostov KE, ter Beest MB, Hansen SH

Epithelial polarity and morphogenesis.

Methods (San Diego, Calif.)

Mostov KE

An ecdysone and tetracycline dual regulatory expression system for studies on Rac1 small GTPase-mediated signaling.

American journal of physiology. Cell physiology

Lai JF, Juang SH, Hung YM, Cheng HY, Cheng TL, Mostov KE, Jou TS

Epithelial polarity and tubulogenesis in vitro.

Trends in cell biology

Zegers MM, O'Brien LE, Yu W, Datta A, Mostov KE

Polarized epithelial membrane traffic: conservation and plasticity.

Nature cell biology

Mostov K, Su T, ter Beest M

The exocyst affects protein synthesis by acting on the translocation machinery of the endoplasmic reticulum.

The Journal of biological chemistry

Lipschutz JH, Lingappa VR, Mostov KE

Cell biology: Just mix and patch.

Nature

Mostov K, Zegers M

Role of rab proteins in epithelial membrane traffic.

International review of cytology

van Ijzendoorn SC, Mostov KE, Hoekstra D

Opinion: Building epithelial architecture: insights from three-dimensional culture models.

Nature reviews. Molecular cell biology

O'Brien LE, Zegers MM, Mostov KE

Exocytosis: the many masters of the exocyst.

Current biology : CB

Lipschutz JH, Mostov KE

Signal transduction. A new thread in an intricate web.

Science (New York, N.Y.)

von Zastrow M, Mostov K

Analysis of membrane traffic in polarized epithelial cells.

Current protocols in cell biology

Lipschutz JH, O'Brien LE, Altschuler Y, Avrahami D, Nguyen Y, Tang K, Mostov KE

Rac1 orientates epithelial apical polarity through effects on basolateral laminin assembly.

Nature cell biology

O'Brien LE, Jou TS, Pollack AL, Zhang Q, Hansen SH, Yurchenco P, Mostov KE

Localization of GFP-tagged concentrative nucleoside transporters in a renal polarized epithelial cell line.

American journal of physiology. Renal physiology

Mangravite LM, Lipschutz JH, Mostov KE, Giacomini KM

Rho GTPase activity modulates Pseudomonas aeruginosa internalization by epithelial cells.

Cellular microbiology

Kazmierczak BI, Jou TS, Mostov K, Engel JN

Interaction of bacterial pathogens with polarized epithelium.

Annual review of microbiology

Kazmierczak BI, Mostov K, Engel JN

Host cell-derived sphingolipids are required for the intracellular growth of Chlamydia trachomatis.

Cellular microbiology

van Ooij C, Kalman L, Nishijima M, Hanada K, Mostov K, Engel JN

Induced expression of Rnd3 is associated with transformation of polarized epithelial cells by the Raf-MEK-extracellular signal-regulated kinase pathway.

Molecular and Cellular Biology

Hansen SH, Zegers MM, Woodrow M, Rodriguez-Viciana P, Chardin P, Mostov KE, McMahon M

The polymeric immunoglobulin receptor translocates pneumococci across human nasopharyngeal epithelial cells.

Cell

Zhang JR, Mostov KE, Lamm ME, Nanno M, Shimida S, Ohwaki M, Tuomanen E

Intracellular redirection of plasma membrane trafficking after loss of epithelial cell polarity.

Molecular biology of the cell

Low SH, Miura M, Roche PA, Valdez AC, Mostov KE, Weimbs T

Membrane traffic in polarized epithelial cells.

Current opinion in cell biology

Mostov KE, Verges M, Altschuler Y

Catch the mu1B train to the basolateral surface.

Cell

Mostov K, ter Beest MB, Chapin SJ

ADP-ribosylation factor 6 and endocytosis at the apical surface of Madin-Darby canine kidney cells.

The Journal of Cell Biology

Altschuler Y, Liu S, Katz L, Tang K, Hardy S, Brodsky F, Apodaca G, Mostov K

The SRC family protein tyrosine kinase p62yes controls polymeric IgA transcytosis in vivo.

Molecular cell

Luton F, Vergés M, Vaerman JP, Sudol M, Mostov KE

A tubular endosomal fraction from rat liver: biochemical evidence of receptor sorting by default.

Proceedings of the National Academy of Sciences of the United States of America

Vergés M, Havel RJ, Mostov KE

Redundant and distinct functions for dynamin-1 and dynamin-2 isoforms.

The Journal of Cell Biology

Altschuler Y, Barbas SM, Terlecky LJ, Tang K, Hardy S, Mostov KE, Schmid SL

Penetration and co-localization in MDCK cell mitochondria of IgA derived from patients with primary biliary cirrhosis.

Journal of autoimmunity

Malmborg AC, Shultz DB, Luton F, Mostov KE, Richly E, Leung PS, Benson GD, Ansari AA, Coppel RL, Gershwin ME, Van de Water J

Susceptibility of epithelial cells to Pseudomonas aeruginosa invasion and cytotoxicity is upregulated by hepatocyte growth factor.

Infection and immunity

Fleiszig SM, Vallas V, Jun CH, Mok L, Balkovetz DF, Roth MG, Mostov KE

The SNARE machinery is involved in apical plasma membrane trafficking in MDCK cells.

The Journal of Cell Biology

Low SH, Chapin SJ, Wimmer C, Whiteheart SW, Kömüves LG, Mostov KE, Weimbs T

Defects in type III secretion correlate with internalization of Pseudomonas aeruginosa by epithelial cells.

Infection and immunity

Hauser AR, Fleiszig S, Kang PJ, Mostov K, Engel JN

Targeting of SNAP-23 and SNAP-25 in polarized epithelial cells.

The Journal of biological chemistry

Low SH, Roche PA, Anderson HA, van Ijzendoorn SC, Zhang M, Mostov KE, Weimbs T

Apical targeting in polarized epithelial cells: There's more afloat than rafts.

Trends in cell biology

Weimbs T, Low SH, Chapin SJ, Mostov KE

Epithelial cell polarity affects susceptibility to Pseudomonas aeruginosa invasion and cytotoxicity.

Infection and immunity

Fleiszig SM, Evans DJ, Do N, Vallas V, Shin S, Mostov KE

Dynamics of beta-catenin interactions with APC protein regulate epithelial tubulogenesis.

The Journal of Cell Biology

Pollack AL, Barth AI, Altschuler Y, Nelson WJ, Mostov KE

Identification of Pseudomonas aeruginosa genes required for epithelial cell injury.

Molecular microbiology

Kang PJ, Hauser AR, Apodaca G, Fleiszig SM, Wiener-Kronish J, Mostov K, Engel JN

Journey across the osteoclast.

Science (New York, N.Y.)

Mostov K, Werb Z

A conserved domain is present in different families of vesicular fusion proteins: a new superfamily.

Proceedings of the National Academy of Sciences of the United States of America

Weimbs T, Low SH, Chapin SJ, Mostov KE, Bucher P, Hofmann K

Hepatocyte growth factor alters the polarity of Madin-Darby canine kidney cell monolayers.

The Journal of biological chemistry

Balkovetz DF, Pollack AL, Mostov KE

Pseudomonas aeruginosa-mediated cytotoxicity and invasion correlate with distinct genotypes at the loci encoding exoenzyme S.

Infection and immunity

Fleiszig SM, Wiener-Kronish JP, Miyazaki H, Vallas V, Mostov KE, Kanada D, Sawa T, Yen TS, Frank DW

Differential localization of syntaxin isoforms in polarized Madin-Darby canine kidney cells.

Molecular biology of the cell

Low SH, Chapin SJ, Weimbs T, Kömüves LG, Bennett MK, Mostov KE

Calmodulin regulates the intracellular trafficking in epithelial cells.

Zeitschrift fur Gastroenterologie

Enrich C, Apodaca G, Mostov KE

Signal transduction by the polymeric immunoglobulin receptor suggests a role in regulation of receptor transcytosis.

The Journal of Cell Biology

Cardone MH, Smith BL, Mennitt PA, Mochly-Rosen D, Silver RB, Mostov KE

Calmodulin binds to the basolateral targeting signal of the polymeric immunoglobulin receptor.

The Journal of biological chemistry

Chapin SJ, Enrich C, Aroeti B, Havel RJ, Mostov KE

Dimeric and tetrameric IgA are transcytosed equally by the polymeric Ig receptor.

Journal of immunology (Baltimore, Md. : 1950)

Song W, Vaerman JP, Mostov KE

Generation and assembly of secretory antibodies in plants.

Science (New York, N.Y.)

Ma JK, Hiatt A, Hein M, Vine ND, Wang F, Stabila P, van Dolleweerd C, Mostov K, Lehner T

Characterization of Pseudomonas aeruginosa-induced MDCK cell injury: glycosylation-defective host cells are resistant to bacterial killing.

Infection and immunity

Apodaca G, Bomsel M, Lindstedt R, Engel J, Frank D, Mostov KE, Wiener-Kronish J

Regulation of protein traffic in polarized epithelial cells.

Histology and histopathology

Mostov KE

Regulation of protein traffic in polarized epithelial cells.

BioEssays : news and reviews in molecular, cellular and developmental biology

Mostov KE, Cardone MH

Regulation of protein traffic in polarized epithelial cells: the polymeric immunoglobulin receptor model.

Cold Spring Harbor symposia on quantitative biology

Mostov KE, Altschuler Y, Chapin SJ, Enrich C, Low SH, Luton F, Richman-Eisenstat J, Singer KL, Tang K, Weimbs T

Rapid internalization of the polymeric immunoglobulin receptor requires phosphorylated serine 726.

The Journal of biological chemistry

Okamoto CT, Song W, Bomsel M, Mostov KE

Receptor-mediated transcytosis of IgA in MDCK cells is via apical recycling endosomes.

The Journal of Cell Biology

Apodaca G, Katz LA, Mostov KE

Targeting of the SF/HGF receptor to the basolateral domain of polarized epithelial cells.

The Journal of Cell Biology

Crepaldi T, Pollack AL, Prat M, Zborek A, Mostov K, Comoglio PM

Phorbol myristate acetate-mediated stimulation of transcytosis and apical recycling in MDCK cells.

The Journal of Cell Biology

Cardone MH, Smith BL, Song W, Mochly-Rosen D, Mostov KE

Stimulation of transcytosis of the polymeric immunoglobulin receptor by dimeric IgA.

Proceedings of the National Academy of Sciences of the United States of America

Song W, Bomsel M, Casanova J, Vaerman JP, Mostov K

Transepithelial transport of immunoglobulins.

Annual review of immunology

Mostov KE

Role of heterotrimeric G proteins in membrane traffic.

Molecular biology of the cell

Bomsel M, Mostov K

Plasma membrane protein sorting in polarized epithelial cells.

The Journal of Cell Biology

Mostov K, Apodaca G, Aroeti B, Okamoto C

Polymeric immunoglobulin receptor.

International review of cytology

Aroeti B, Casanova J, Okamoto C, Cardone M, Pollack A, Tang K, Mostov K

Direct apical sorting of rat liver dipeptidylpeptidase IV expressed in Madin-Darby canine kidney cells.

The Journal of biological chemistry

Casanova JE, Mishumi Y, Ikehara Y, Hubbard AL, Mostov KE

The polymeric immunoglobulin receptor.

Seminars in cell biology

Mostov K

Sorting of plasma membrane proteins in epithelial cells.

Current opinion in cell biology

Bomsel M, Mostov K

The polymeric immunoglobulin receptor. A model protein to study transcytosis.

The Journal of clinical investigation

Apodaca G, Bomsel M, Arden J, Breitfeld PP, Tang K, Mostov KE

Phosphorylation of the polymeric immunoglobulin receptor required for its efficient transcytosis.

Science (New York, N.Y.)

Casanova JE, Breitfeld PP, Ross SA, Mostov KE

Vectorial targeting of an endogenous apical membrane sialoglycoprotein and uvomorulin in MDCK cells.

The Journal of Cell Biology

Le Bivic A, Sambuy Y, Mostov K, Rodriguez-Boulan E

Transepithelial transport of immunoglobulins: a model of protein sorting and transcytosis.

American journal of respiratory cell and molecular biology

Breitfeld PP, Casanova JE, Simister NE, Ross SA, McKinnon WC, Mostov KE

Sorting signals.

Current opinion in cell biology

Breitfeld PP, Casanova JE, Simister NE, Ross SA, McKinnon WC, Mostov KE

Expression and analysis of the polymeric immunoglobulin receptor in Madin-Darby canine kidney cells using retroviral vectors.

Methods in cell biology

Breitfeld PP, Casanova JE, Harris JM, Simister NE, Mostov KE

Cell biology of the IgA receptor in polarized epithelia.

Monographs in allergy

Breitfeld PP, Harris JM, Mostov KE

Alternate splicing of rabbit polymeric immunoglobulin receptor.

Molecular and Cellular Biology

Deitcher DL, Mostov KE

Structure and function of the receptor for polymeric immunoglobulins.

Biochemical Society symposium

Mostov KE, Friedlander M, Blobel G

Transcytosis.

Cell

Mostov KE, Simister NE

Biosynthesis, processing, and function of secretory component.

Methods in enzymology

Mostov KE, Blobel G

In vitro translation and processing of a precursor form of favin, a lectin from Vicia faba.

The Journal of biological chemistry

Hemperly JJ, Mostov KE, Cunningham BA

Receptor-mediated transcellular transport of immunoglobulin: synthesis of secretory component as multiple and larger transmembrane forms.

Proceedings of the National Academy of Sciences of the United States of America

Mostov KE, Kraehenbuhl JP, Blobel G

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