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Lei Wang, PhD

Professor
Pharmaceutical Chemistry
Address: 
555 Mission Bay Blvd South, Rm 452X
UCSF Box 3122
San Francisco, CA 94158
United States
Phone: 
+1 415 502-3383
Email: 
[email protected]
UCSF Profiles
Research Interests: 
Biospecific chemistry and covalent biotherapeutics

Research Summary:
We build proteins in living cells using new amino acids. By harnessing the novel properties of these new building blocks, we probe biological processes in their natural settings and engineer unique biomolecules to understand mechanisms of cellular function and to develop new treatments of diseases.

Publications: 

Identification of Covalent Cyclic Peptide Inhibitors Targeting Protein-Protein Interactions Using Phage Display.

Journal of the American Chemical Society

Wang S, Faucher FF, Bertolini M, Kim H, Yu B, Cao L, Roeltgen K, Lovell S, Shanker V, Boyd SD, Wang L, Bartenschlager R, Bogyo M

Arginine Accelerates Sulfur Fluoride Exchange and Phosphorus Fluoride Exchange Reactions between Proteins.

Angewandte Chemie (International ed. in English)

Cao L, Yu B, Klauser P, Zhang P, Li S, Wang L

The proximity-enabled sulfur fluoride exchange reaction in the protein context.

Chemical science

Yu B, Cao L, Li S, Klauser PC, Wang L

Covalent Proteins as Targeted Radionuclide Therapies Enhance Antitumor Effects.

ACS central science

Klauser PC, Chopra S, Cao L, Bobba KN, Yu B, Seo Y, Chan E, Flavell RR, Evans MJ, Wang L

Genetically encoded chemical crosslinking of carbohydrate.

Nature chemistry

Li S, Wang N, Yu B, Sun W, Wang L

Genetically encoded chemical crosslinking of RNA in vivo.

Nature chemistry

Sun W, Wang N, Liu H, Yu B, Jin L, Ren X, Shen Y, Wang L

Accelerating PERx reaction enables covalent nanobodies for potent neutralization of SARS-CoV-2 and variants.

Chem

Yu B, Li S, Tabata T, Wang N, Cao L, Kumar GR, Sun W, Liu J, Ott M, Wang L

Encoding latent SuFEx reactive meta-fluorosulfate tyrosine to expand covalent bonding of proteins.

Chemical communications (Cambridge, England)

Klauser PC, Berdan VY, Cao L, Wang L

Accelerating PERx Reaction Enables Covalent Nanobodies for Potent Neutralization of SARS-Cov-2 and Variants.

bioRxiv : the preprint server for biology

Yu B, Li S, Tabata T, Wang N, Kumar GR, Liu J, Ott MM, Wang L

New covalent bonding ability for proteins.

Protein science : a publication of the Protein Society

Cao L, Wang L

A Genetically Encoded Fluorosulfonyloxybenzoyl-l-lysine for Expansive Covalent Bonding of Proteins via SuFEx Chemistry.

Journal of the American Chemical Society

Liu J, Cao L, Klauser PC, Cheng R, Berdan VY, Sun W, Wang N, Ghelichkhani F, Yu B, Rozovsky S, Wang L

Genetic Incorporation of ϵ-N-Benzoyllysine by Engineering Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase.

Chembiochem : a European journal of chemical biology

Cao L, Liu J, Ghelichkhani F, Rozovsky S, Wang L

Covalent peptides and proteins for therapeutics.

Bioorganic & medicinal chemistry

Berdan VY, Klauser PC, Wang L

Targeting Phosphotyrosine in Native Proteins with Conditional, Bispecific Antibody Traps.

Journal of the American Chemical Society

Zhou XX, Bracken CJ, Zhang K, Zhou J, Mou Y, Wang L, Cheng Y, Leung KK, Wells JA

Genetically Encoded Quinone Methides Enabling Rapid, Site-Specific, and Photocontrolled Protein Modification with Amine Reagents.

Journal of the American Chemical Society

Liu J, Cheng R, Van Eps N, Wang N, Morizumi T, Ou WL, Klauser PC, Rozovsky S, Ernst OP, Wang L

Genetically encoding thyronine for fluorescent detection of peroxynitrite.

Bioorganic & medicinal chemistry

Li S, Yang B, Kobayashi T, Yu B, Liu J, Wang L

Developing Covalent Protein Drugs via Proximity-Enabled Reactive Therapeutics.

Cell

Li Q, Chen Q, Klauser PC, Li M, Zheng F, Wang N, Li X, Zhang Q, Fu X, Wang Q, Xu Y, Wang L

Synthesis of Chiral Spin-Labeled Amino Acids.

Organic letters

Vuong W, Mosquera-Guagua F, Sanichar R, McDonald TR, Ernst OP, Wang L, Vederas JC

Photocaged Quinone Methide Crosslinkers for Light-Controlled Chemical Crosslinking of Protein-Protein and Protein-DNA Complexes.

Angewandte Chemie (International ed. in English)

Liu J, Cai L, Sun W, Cheng R, Wang N, Jin L, Rozovsky S, Seiple IB, Wang L

Genetically Encoding Photocaged Quinone Methide to Multitarget Protein Residues Covalently in Vivo.

Journal of the American Chemical Society

Liu J, Li S, Aslam NA, Zheng F, Yang B, Cheng R, Wang N, Rozovsky S, Wang PG, Wang Q, Wang L

Genetically Introducing Biochemically Reactive Amino Acids Dehydroalanine and Dehydrobutyrine in Proteins.

Journal of the American Chemical Society

Yang B, Wang N, Schnier PD, Zheng F, Zhu H, Polizzi NF, Ittuveetil A, Saikam V, DeGrado WF, Wang Q, Wang PG, Wang L

Proximity-enhanced SuFEx chemical cross-linker for specific and multitargeting cross-linking mass spectrometry.

Proceedings of the National Academy of Sciences of the United States of America

Yang B, Wu H, Schnier PD, Liu Y, Liu J, Wang N, DeGrado WF, Wang L

Building and Breaking Bonds via a Compact S-Propargyl-Cysteine to Chemically Control Enzymes and Modify Proteins.

Angewandte Chemie (International ed. in English)

Liu J, Cheng R, Wu H, Li S, Wang PG, DeGrado WF, Rozovsky S, Wang L

Genetically Encoding Quinoline Reverses Chromophore Charge and Enables Fluorescent Protein Brightening in Acidic Vesicles.

Journal of the American Chemical Society

Fu C, Kobayashi T, Wang N, Hoppmann C, Yang B, Irannejad R, Wang L

Site-Specific Incorporation of Selenocysteine Using an Expanded Genetic Code and Palladium-Mediated Chemical Deprotection.

Journal of the American Chemical Society

Liu J, Zheng F, Cheng R, Li S, Rozovsky S, Wang Q, Wang L

Genetically Encoding Fluorosulfate-l-tyrosine To React with Lysine, Histidine, and Tyrosine via SuFEx in Proteins in Vivo.

Journal of the American Chemical Society

Wang N, Yang B, Fu C, Zhu H, Zheng F, Kobayashi T, Liu J, Li S, Ma C, Wang PG, Wang Q, Wang L

Spontaneous and specific chemical cross-linking in live cells to capture and identify protein interactions.

Nature communications

Yang B, Tang S, Ma C, Li ST, Shao GC, Dang B, DeGrado WF, Dong MQ, Wang PG, Ding S, Wang L

Site-specific incorporation of phosphotyrosine using an expanded genetic code.

Nature chemical biology

Hoppmann C, Wong A, Yang B, Li S, Hunter T, Shokat KM, Wang L

Optocontrol of glutamate receptor activity by single side-chain photoisomerization.

eLife

Klippenstein V, Hoppmann C, Ye S, Wang L, Paoletti P

Heritable expansion of the genetic code in mouse and zebrafish.

Cell research

Chen Y, Ma J, Lu W, Tian M, Thauvin M, Yuan C, Volovitch M, Wang Q, Holst J, Liu M, Vriz S, Ye S, Wang L, Li D

Using Protein-Confined Proximity To Determine Chemical Reactivity.

Journal of the American Chemical Society

Kobayashi T, Hoppmann C, Yang B, Wang L

Genetically encoding new bioreactivity.

New biotechnology

Wang L

Cysteine Sulfoxidation Increases the Photostability of Red Fluorescent Proteins.

ACS chemical biology

Ren H, Yang B, Ma C, Hu YS, Wang PG, Wang L

Development of background-free tame fluorescent probes for intracellular live cell imaging.

Nature communications

Alamudi SH, Satapathy R, Kim J, Su D, Ren H, Das R, Hu L, Alvarado-Martínez E, Lee JY, Hoppmann C, Peña-Cabrera E, Ha HH, Park HS, Wang L, Chang YT

Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons.

Journal of visualized experiments : JoVE

Kang JY, Kawaguchi D, Wang L

Proximity-enabled bioreactivity to generate covalent peptide inhibitors of p53-Mdm4.

Chemical communications (Cambridge, England)

Hoppmann C, Wang L

In Situ Formation of an Azo Bridge on Proteins Controllable by Visible Light.

Journal of the American Chemical Society

Hoppmann C, Maslennikov I, Choe S, Wang L

A versatile platform to analyze low-affinity and transient protein-protein interactions in living cells in real time.

Cell reports

Li YC, Rodewald LW, Hoppmann C, Wong ET, Lebreton S, Safar P, Patek M, Wang L, Wertman KF, Wahl GM

Response and adaptation of Escherichia coli to suppression of the amber stop codon.

Chembiochem : a European journal of chemical biology

Wang Q, Sun T, Xu J, Shen Z, Briggs SP, Zhou D, Wang L

Genetically encoding photoswitchable click amino acids in Escherichia coli and mammalian cells.

Angewandte Chemie (International ed. in English)

Hoppmann C, Lacey VK, Louie GV, Wei J, Noel JP, Wang L

Proximity-enabled protein crosslinking through genetically encoding haloalkane unnatural amino acids.

Angewandte Chemie (International ed. in English)

Xiang Z, Lacey VK, Ren H, Xu J, Burban DJ, Jennings PA, Wang L

Genetically encoded chemical probes in cells reveal the binding path of urocortin-I to CRF class B GPCR.

Cell

Coin I, Katritch V, Sun T, Xiang Z, Siu FY, Beyermann M, Stevens RC, Wang L

In vivo expression of a light-activatable potassium channel using unnatural amino acids.

Neuron

Kang JY, Kawaguchi D, Coin I, Xiang Z, O'Leary DD, Slesinger PA, Wang L

Adding an unnatural covalent bond to proteins through proximity-enhanced bioreactivity.

Nature methods

Xiang Z, Ren H, Hu YS, Coin I, Wei J, Cang H, Wang L

Expression of fluorescent cyclotides using protein trans-splicing for easy monitoring of cyclotide-protein interactions.

Angewandte Chemie (International ed. in English)

Jagadish K, Borra R, Lacey V, Majumder S, Shekhtman A, Wang L, Camarero JA

Release factor one is nonessential in Escherichia coli.

ACS chemical biology

Johnson DB, Wang C, Xu J, Schultz MD, Schmitz RJ, Ecker JR, Wang L

Expanding the genetic code of Caenorhabditis elegans using bacterial aminoacyl-tRNA synthetase/tRNA pairs.

ACS chemical biology

Parrish AR, She X, Xiang Z, Coin I, Shen Z, Briggs SP, Dillin A, Wang L

Genetic incorporation of unnatural amino acids into proteins in yeast.

Methods in molecular biology (Clifton, N.J.)

Wang Q, Wang L

RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites.

Nature chemical biology

Johnson DB, Xu J, Shen Z, Takimoto JK, Schultz MD, Schmitz RJ, Xiang Z, Ecker JR, Briggs SP, Wang L

A fluorescent reporter of the phosphorylation status of the substrate protein STAT3.

Angewandte Chemie (International ed. in English)

Lacey VK, Parrish AR, Han S, Shen Z, Briggs SP, Ma Y, Wang L

Photo-cross-linkers incorporated into G-protein-coupled receptors in mammalian cells: a ligand comparison.

Angewandte Chemie (International ed. in English)

Coin I, Perrin MH, Vale WW, Wang L

Genetic Incorporation of Unnatural Amino Acids into Proteins

Comprehensive Natural Products Chemistry II Chemistry and Biology

Parrish AR, Wang L

Imprints of the genetic code in the ribosome.

Proceedings of the National Academy of Sciences of the United States of America

Johnson DB, Wang L

Impact of nonnatural amino acid mutagenesis on the in vivo function and binding modes of a transcriptional activator.

Journal of the American Chemical Society

Majmudar CY, Lee LW, Lancia JK, Nwokoye A, Wang Q, Wands AM, Wang L, Mapp AK

Expanding the genetic code for biological studies.

Chemistry & biology

Wang Q, Parrish AR, Wang L

Unique interactions between the chromophore and glutamate 16 lead to far-red emission in a red fluorescent protein.

Protein science : a publication of the Protein Society

Shu X, Wang L, Colip L, Kallio K, Remington SJ

Unnatural Amino Acids to Investigate Biological Processes

Wiley Encyclopedia of Chemical Biology

Johnson DBF, Takimoto JK, Xu J, Wang L

New methods enabling efficient incorporation of unnatural amino acids in yeast.

Journal of the American Chemical Society

Wang Q, Wang L

Genetically encoding unnatural amino acids for cellular and neuronal studies.

Nature neuroscience

Wang W, Takimoto JK, Louie GV, Baiga TJ, Noel JP, Lee KF, Slesinger PA, Wang L

Manipulating proteins for neuroscience.

Current opinion in neurobiology

Parrish AR, Wang W, Wang L

Expanding the genetic code.

Annual review of biophysics and biomolecular structure

Wang L, Xie J, Schultz PG

Crystal structures of apo wild-type M. jannaschii tyrosyl-tRNA synthetase (TyrRS) and an engineered TyrRS specific for O-methyl-L-tyrosine.

Protein science : a publication of the Protein Society

Zhang Y, Wang L, Schultz PG, Wilson IA

Expanding the genetic code.

Angewandte Chemie (International ed. in English)

Wang L, Schultz PG

Evolution of new nonantibody proteins via iterative somatic hypermutation.

Proceedings of the National Academy of Sciences of the United States of America

Wang L, Jackson WC, Steinbach PA, Tsien RY

The site-specific incorporation of p-iodo-L-phenylalanine into proteins for structure determination.

Nature biotechnology

Xie J, Wang L, Wu N, Brock A, Spraggon G, Schultz PG

A new strategy for the site-specific modification of proteins in vivo.

Biochemistry

Zhang Z, Smith BA, Wang L, Brock A, Cho C, Schultz PG

A method for the generation of glycoprotein mimetics.

Journal of the American Chemical Society

Liu H, Wang L, Brock A, Wong CH, Schultz PG

Generation of a bacterium with a 21 amino acid genetic code.

Journal of the American Chemical Society

Mehl RA, Anderson JC, Santoro SW, Wang L, Martin AB, King DS, Horn DM, Schultz PG

Unnatural amino acid mutagenesis of green fluorescent protein.

The Journal of organic chemistry

Wang L, Xie J, Deniz AA, Schultz PG

Addition of the keto functional group to the genetic code of Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America

Wang L, Zhang Z, Brock A, Schultz PG

An efficient system for the evolution of aminoacyl-tRNA synthetase specificity.

Nature biotechnology

Santoro SW, Wang L, Herberich B, King DS, Schultz PG

Addition of p-azido-L-phenylalanine to the genetic code of Escherichia coli.

Journal of the American Chemical Society

Chin JW, Santoro SW, Martin AB, King DS, Wang L, Schultz PG

The selective incorporation of alkenes into proteins in Escherichia coli.

Angewandte Chemie (International ed. in English)

Zhang Z, Wang L, Brock A, Schultz PG

Addition of a photocrosslinking amino acid to the genetic code of Escherichiacoli.

Proceedings of the National Academy of Sciences of the United States of America

Chin JW, Martin AB, King DS, Wang L, Schultz PG

Adding L-3-(2-Naphthyl)alanine to the genetic code of E. coli.

Journal of the American Chemical Society

Wang L, Brock A, Schultz PG

Expanding the genetic code.

Chemical communications (Cambridge, England)

Wang L, Schultz PG

A general approach for the generation of orthogonal tRNAs.

Chemistry & biology

Wang L, Schultz PG

Expanding the genetic code of Escherichia coli.

Science (New York, N.Y.)

Wang L, Brock A, Herberich B, Schultz PG

Electrophysiological properties of the recipient atrial remnant after human orthotopic cardiac transplantation.

Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology

Holt ND, Hetherington K, Brady S, Dark JH, McComb JM

Developing High Resolution Electrical Probing System Based on Atomic Force Microscopy

Molecular Crystals and Liquid Crystals

Chen Y, Wang L, He HX, Liu ZF

Fabrication of Gold Nanostructures on Graphite Using Atomic Force Microscope

Molecular Crystals and Liquid Crystals

Song JQ, Li CZ, He HX, Chen Y, Wang L, Liu ZF

Force Titration of a Carboxylic Acid Terminated Self-Assemble Monolayer Using Chemical Force Microscopy

Molecular Crystals and Liquid Crystals

He HX, Li CZ, Song JQ, Mu T, Wang L, Zhang HL, Liu ZF

NOTE

Journal of colloid and interface science

Zhu B, Zhang P, Wang L, Liu Z

Websites: 
https://pharm.ucsf.edu/wang
Faculty Type: 
Core CVRI Faculty