Professor
Pharmaceutical Chemistry
Research Interests
Biospecific chemistry and covalent biotherapeutics
Email
+1 415 502-3383
Websites
Faculty Type
Core CVRI Faculty
Research Summary:
We build proteins in living cells using new amino acids. By harnessing the novel properties of these new building blocks, we probe biological processes in their natural settings and engineer unique biomolecules to understand mechanisms of cellular function and to develop new treatments of diseases.
Publications
Identification of Covalent Cyclic Peptide Inhibitors Targeting Protein-Protein Interactions Using Phage Display.
Journal of the American Chemical Society
Arginine Accelerates Sulfur Fluoride Exchange and Phosphorus Fluoride Exchange Reactions between Proteins.
Angewandte Chemie (International ed. in English)
Biospecific Chemistry for Covalent Linking of Biomacromolecules.
Chemical reviews
Covalent Proteins as Targeted Radionuclide Therapies Enhance Antitumor Effects.
ACS central science
Residue selective crosslinking of proteins through photoactivatable or proximity-enabled reactivity.
Current opinion in chemical biology
Genetically encoded chemical crosslinking of carbohydrate.
Nature chemistry
Genetically encoded chemical crosslinking of RNA in vivo.
Nature chemistry
Encoding latent SuFEx reactive meta-fluorosulfate tyrosine to expand covalent bonding of proteins.
Chemical communications (Cambridge, England)
Accelerating PERx Reaction Enables Covalent Nanobodies for Potent Neutralization of SARS-Cov-2 and Variants.
bioRxiv : the preprint server for biology
Genetically encoding latent bioreactive amino acids and the development of covalent protein drugs.
Current opinion in chemical biology
New covalent bonding ability for proteins.
Protein science : a publication of the Protein Society
A Genetically Encoded Fluorosulfonyloxybenzoyl-l-lysine for Expansive Covalent Bonding of Proteins via SuFEx Chemistry.
Journal of the American Chemical Society
Genetic Incorporation of ϵ-N-Benzoyllysine by Engineering Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase.
Chembiochem : a European journal of chemical biology
Covalent peptides and proteins for therapeutics.
Bioorganic & medicinal chemistry
Targeting Phosphotyrosine in Native Proteins with Conditional, Bispecific Antibody Traps.
Journal of the American Chemical Society
Genetically Encoded Quinone Methides Enabling Rapid, Site-Specific, and Photocontrolled Protein Modification with Amine Reagents.
Journal of the American Chemical Society
Genetically encoding thyronine for fluorescent detection of peroxynitrite.
Bioorganic & medicinal chemistry
Acid-brightening fluorescent protein (abFP) for imaging acidic vesicles and organelles.
Methods in enzymology
Synthesis of Chiral Spin-Labeled Amino Acids.
Organic letters
Photocaged Quinone Methide Crosslinkers for Light-Controlled Chemical Crosslinking of Protein-Protein and Protein-DNA Complexes.
Angewandte Chemie (International ed. in English)
Genetically Encoding Photocaged Quinone Methide to Multitarget Protein Residues Covalently in Vivo.
Journal of the American Chemical Society
Genetically Introducing Biochemically Reactive Amino Acids Dehydroalanine and Dehydrobutyrine in Proteins.
Journal of the American Chemical Society
Genetically encoding photoswitchable click amino acids for general optical control of conformation and function of proteins.
Methods in enzymology
Proximity-enhanced SuFEx chemical cross-linker for specific and multitargeting cross-linking mass spectrometry.
Proceedings of the National Academy of Sciences of the United States of America
Building and Breaking Bonds via a Compact S-Propargyl-Cysteine to Chemically Control Enzymes and Modify Proteins.
Angewandte Chemie (International ed. in English)
Genetically Encoding Quinoline Reverses Chromophore Charge and Enables Fluorescent Protein Brightening in Acidic Vesicles.
Journal of the American Chemical Society
Site-Specific Incorporation of Selenocysteine Using an Expanded Genetic Code and Palladium-Mediated Chemical Deprotection.
Journal of the American Chemical Society
Genetically Encoding Fluorosulfate-l-tyrosine To React with Lysine, Histidine, and Tyrosine via SuFEx in Proteins in Vivo.
Journal of the American Chemical Society
Genetically Encoding Unnatural Amino Acids in Neurons In Vitro and in the Embryonic Mouse Brain for Optical Control of Neuronal Proteins.
Methods in molecular biology (Clifton, N.J.)
Spontaneous and specific chemical cross-linking in live cells to capture and identify protein interactions.
Nature communications
Engineering the Genetic Code in Cells and Animals: Biological Considerations and Impacts.
Accounts of chemical research
Site-specific incorporation of phosphotyrosine using an expanded genetic code.
Nature chemical biology
Using Protein-Confined Proximity To Determine Chemical Reactivity.
Journal of the American Chemical Society
Editorial overview: Chemistry for biopolymers to investigate and even move beyond nature.
Current opinion in chemical biology
Genetically encoding new bioreactivity.
New biotechnology
Cysteine Sulfoxidation Increases the Photostability of Red Fluorescent Proteins.
ACS chemical biology
Development of background-free tame fluorescent probes for intracellular live cell imaging.
Nature communications
Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons.
Journal of visualized experiments : JoVE
Proximity-enabled bioreactivity to generate covalent peptide inhibitors of p53-Mdm4.
Chemical communications (Cambridge, England)
In Situ Formation of an Azo Bridge on Proteins Controllable by Visible Light.
Journal of the American Chemical Society
A versatile platform to analyze low-affinity and transient protein-protein interactions in living cells in real time.
Cell reports
Genetically encoding an electrophilic amino acid for protein stapling and covalent binding to native receptors.
ACS chemical biology
Response and adaptation of Escherichia coli to suppression of the amber stop codon.
Chembiochem : a European journal of chemical biology
Genetically encoding photoswitchable click amino acids in Escherichia coli and mammalian cells.
Angewandte Chemie (International ed. in English)
Proximity-enabled protein crosslinking through genetically encoding haloalkane unnatural amino acids.
Angewandte Chemie (International ed. in English)
Expanding the library and substrate diversity of the pyrrolysyl-tRNA synthetase to incorporate unnatural amino acids containing conjugated rings.
Chembiochem : a European journal of chemical biology
Adding an unnatural covalent bond to proteins through proximity-enhanced bioreactivity.
Nature methods
Expression of fluorescent cyclotides using protein trans-splicing for easy monitoring of cyclotide-protein interactions.
Angewandte Chemie (International ed. in English)
Release factor one is nonessential in Escherichia coli.
ACS chemical biology
Expanding the genetic code of Caenorhabditis elegans using bacterial aminoacyl-tRNA synthetase/tRNA pairs.
ACS chemical biology
Genetic incorporation of unnatural amino acids into proteins in yeast.
Methods in molecular biology (Clifton, N.J.)
RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites.
Nature chemical biology
Genetically encoding unnatural amino acids in neural stem cells and optically reporting voltage-sensitive domain changes in differentiated neurons.
Stem cells (Dayton, Ohio)
A fluorescent reporter of the phosphorylation status of the substrate protein STAT3.
Angewandte Chemie (International ed. in English)
Photo-cross-linkers incorporated into G-protein-coupled receptors in mammalian cells: a ligand comparison.
Angewandte Chemie (International ed. in English)
Enantiospecific synthesis of genetically encodable fluorescent unnatural amino acid L-3-(6-acetylnaphthalen-2-ylamino)-2-aminopropanoic acid.
The Journal of organic chemistry
Stereochemical basis for engineered pyrrolysyl-tRNA synthetase and the efficient in vivo incorporation of structurally divergent non-native amino acids.
ACS chemical biology
Genetic Incorporation of Unnatural Amino Acids into Proteins
Comprehensive Natural Products Chemistry II Chemistry and Biology
Esterification of an unnatural amino acid structurally deviating from canonical amino acids promotes its uptake and incorporation into proteins in mammalian cells.
Chembiochem : a European journal of chemical biology
Imprints of the genetic code in the ribosome.
Proceedings of the National Academy of Sciences of the United States of America
Impact of nonnatural amino acid mutagenesis on the in vivo function and binding modes of a transcriptional activator.
Journal of the American Chemical Society
Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells.
Molecular bioSystems
Expanding the genetic code for biological studies.
Chemistry & biology
Unique interactions between the chromophore and glutamate 16 lead to far-red emission in a red fluorescent protein.
Protein science : a publication of the Protein Society
Selective labeling of proteins with chemical probes in living cells.
Physiology (Bethesda, Md.)
Unnatural Amino Acids to Investigate Biological Processes
Wiley Encyclopedia of Chemical Biology
New methods enabling efficient incorporation of unnatural amino acids in yeast.
Journal of the American Chemical Society
Genetically encoding unnatural amino acids for cellular and neuronal studies.
Nature neuroscience
Manipulating proteins for neuroscience.
Current opinion in neurobiology
Evolving proteins in mammalian cells using somatic hypermutation.
Nature protocols
Expanding the genetic code.
Annual review of biophysics and biomolecular structure
Crystal structures of apo wild-type M. jannaschii tyrosyl-tRNA synthetase (TyrRS) and an engineered TyrRS specific for O-methyl-L-tyrosine.
Protein science : a publication of the Protein Society
Expanding the genetic code.
Angewandte Chemie (International ed. in English)
Evolution of new nonantibody proteins via iterative somatic hypermutation.
Proceedings of the National Academy of Sciences of the United States of America
The site-specific incorporation of p-iodo-L-phenylalanine into proteins for structure determination.
Nature biotechnology
Amersham Prize winner. Expanding the genetic code.
Science (New York, N.Y.)
A method for the generation of glycoprotein mimetics.
Journal of the American Chemical Society
Generation of a bacterium with a 21 amino acid genetic code.
Journal of the American Chemical Society
Unnatural amino acid mutagenesis of green fluorescent protein.
The Journal of organic chemistry
Addition of the keto functional group to the genetic code of Escherichia coli.
Proceedings of the National Academy of Sciences of the United States of America
An efficient system for the evolution of aminoacyl-tRNA synthetase specificity.
Nature biotechnology
Addition of p-azido-L-phenylalanine to the genetic code of Escherichia coli.
Journal of the American Chemical Society
The selective incorporation of alkenes into proteins in Escherichia coli.
Angewandte Chemie (International ed. in English)
Addition of a photocrosslinking amino acid to the genetic code of Escherichiacoli.
Proceedings of the National Academy of Sciences of the United States of America
Adding L-3-(2-Naphthyl)alanine to the genetic code of E. coli.
Journal of the American Chemical Society
Expanding the genetic code.
Chemical communications (Cambridge, England)
A general approach for the generation of orthogonal tRNAs.
Chemistry & biology
Expanding the genetic code of Escherichia coli.
Science (New York, N.Y.)
Electrophysiological properties of the recipient atrial remnant after human orthotopic cardiac transplantation.
Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology
Developing High Resolution Electrical Probing System Based on Atomic Force Microscopy
Molecular Crystals and Liquid Crystals
Fabrication of Gold Nanostructures on Graphite Using Atomic Force Microscope
Molecular Crystals and Liquid Crystals
Force Titration of a Carboxylic Acid Terminated Self-Assemble Monolayer Using Chemical Force Microscopy
Molecular Crystals and Liquid Crystals
NOTE
Journal of colloid and interface science
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